بررسی مولکولی گونه های بیماریزای آسپرژیلوس جدا شده زخم پای افراد دیابتی با استفاده از روش Nested PCR
Authors
Abstract:
Background and Aim: PCR has high sensitivity and specificity for the determination offungal DNA. It is also useful for rapid identification of the most common species of Aspergillus. The purpose of this study was to compare culture method with nested PCR method to determine Aspergillesion in diabetic foot patient in Imam Khomeini Hospital, Tehran. Material and Methods: Sixty-five cases of suspicious diabetic foot lesion Aspergillusis were examined under culture and molecular method. Aspergillus isolates were identified to genus level on the SGA. DNA extraction performed and PCR and Nested PCR reactions were done. Serum samples were collected and ELISA for anti bodies against all species were performed. Results were analyzed using ANOVA and P was considered significant at P<0.05. Results: Of the total of 65 samples, 39 were diagnosed as Aspergillos is obtained from cultures. In PCR and Nested PCR using specific primers, 41 samples were diagnosed as Aspergillus.PCR and Nested PCR for species recognition were equal but differences in gender were recognized. ELISA results showed that 39 samples were positive. Conclusion: Although PCR is less sensitive than nested PCR, but we can recognize the most common pathogenic species, Aspergillus fumigatus. Our results showed significant differences between the results of culture and nested-PCR in the identification of the Aspergillus genus.
similar resources
بررسی مولکولی گونه های بیماریزای آسپرژیلوس جدا شده زخم پای افراد دیابتی با استفاده از روش nested pcr
زمینه و هدف: روش pcr حساسیت و ویژگی بالایی را جهت تعیین dna قارچی و شناسایی سریع اغلب گونه های شایع کلینیکی آسپرژیلوس را ارائه میدهد. هدف از این مطالعه مقایسه کشت با روش nestedpcr برای تعیین آسپرژیلوزیس از زخم پای بیماران دیابتی مراجعه کننده به بیمارستان امام خمینی تهران بود. روش: 65 نمونه زخم های مشکوک به آسپرزیلوزیس موجود در زخم پای دیابتی تحت شرایط کشت و pcr قرار گرفتند. ایزوله های آسپرژیل...
full textاثر بربرین در تنظیم آستروسیتهای Gfap+ ناحیه هیپوکمپ موشهای صحرایی دیابتی شده با استرپتوزوتوسین
Background: Diabetes mellitus increases the risk of central nervous system (CNS) disorders such as stroke, seizures, dementia, and cognitive impairment. Berberine, a natural isoquinolne alkaloid, is reported to exhibit beneficial effect in various neurodegenerative and neuropsychiatric disorders. Moreover astrocytes are proving critical for normal CNS function, and alterations in their activity...
full textاثر بربرین در تنظیم آستروسیتهای Gfap+ ناحیه هیپوکمپ موشهای صحرایی دیابتی شده با استرپتوزوتوسین
Background: Diabetes mellitus increases the risk of central nervous system (CNS) disorders such as stroke, seizures, dementia, and cognitive impairment. Berberine, a natural isoquinolne alkaloid, is reported to exhibit beneficial effect in various neurodegenerative and neuropsychiatric disorders. Moreover astrocytes are proving critical for normal CNS function, and alterations in their activity...
full textشناسایی گونه های کاندیدا از افراد دیابتی با استفاده از روش PCR-RFLP
Abstract Background and purpose: Diabetes affects the function of many organs in the body. It predisposes an environment for colonization of different microorganisms including Candida species which are normal floral of the skin, vagina and digestive system and increases their susceptibility to fungal infections. This study aimed at isolating different species of candida in diabetic patients ...
full textتفریق مولکولی آسپرژیلوس های جدا شده از هوای تهران با استفاده از تکنیک rapd-pcr
در مطالعه حاضر، تنوع ژنتیکی و روابط فیلوژنی گونه های آسپرژیلوس جداسازی شده از هوای تهران با استفاده از روش مولکولی تکثیر تصادفی dna چندشکلی (rapd-pcr)مورد بررسی قرار گرفت. 38 ایزوله از 12 گونه آسپرژیلوس، شامل 11 ایزوله از آسپرژیلوس نایجر، 7 ایزوله از آسپرژیلوس فلاووس، 4 ایزوله از آسپرژیلوس ژاپونیکوس، 5 ایزوله از آسپرژیلوس توبینجنسیس، 4 ایزوله از آسپرژیلوس اوخراسئوس و 1 ایزوله از هر یک از گونه...
My Resources
Journal title
volume 1 issue 4
pages 25- 32
publication date 2014-01
By following a journal you will be notified via email when a new issue of this journal is published.
No Keywords
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023